(二)荧光显微镜技术 特异蛋白质等生物大分子定性定位的最有力的工具。 Barrier filter Exciter matic A Figure 4-12 The epi-fluorescence microscopc. Light from a multiwavelength source moves through an exciter Filter, which allows only the desired wavelength of exciting radia- tion to pass. This radiation is reflected by the dichromatic filer and focused by the objective lens onto the sample; luo. Objective lens rescent molecules in the sample arc then excited to emit light oresce)at a specific and longer wavelength focuscd by the objective lens; most of it passes through che Specimen dichromatic filter and is not reflected. A final barrier filter blocks any residual light with the frequcncy of the exciting（二）荧光显微镜技术 特异蛋白质等生物大分子定性定位的最有力的工具