Question 2, continued c)Suppose that you are studying the interactions between the substrate and the enzyme. It is possible to make variant enzymes that differ from the one above by a single amino acid substitution (For example, Asp 78 could be replaced with tryptophan). you could use this technique to investigate the roles of each amino acid shown above i)If you change Arg 31 to a lysine, would you predict that the substrate still binds, or that the substrate now fails to bind to the altered enzyme? Explain. Choose an amino acid substitution for ser 161 ii) Explain the possible outcome of this change Note: There are many possible full-credit answers for (i) and (ii). a table of amino acid structures can be found at the end of this exam Question 3 a)Brief ly define the following Dominant Recessive Phenotype enotype alleles Homozygous Heterozygous Mendel's first Law: Mendel,s second Law Diploid b)A yeast cell has the genotype AaBb, where the a and the b loci are on different chromosomes. Sketch the chromosome arrangement of this cell in meiosis I, when 2n 4 44 Question 2, continued c) Suppose that you are studying the interactions between the substrate and the enzyme. It is possible to make variant enzymes that differ from the one above by a single amino acid substitution. (For example, Asp 78 could be replaced with tryptophan). You could use this technique to investigate the roles of each amino acid shown above. i) If you change Arg 31 to a lysine, would you predict that the substrate still binds, or that the substrate now fails to bind to the altered enzyme? Explain. ii) Choose an amino acid substitution for Ser 161. iii) Explain the possible outcome of this change. Note: There are many possible full-credit answers for (ii) and (iii). A table of amino acid structures can be found at the end of this exam. Question 3 a) Briefly define the following: Dominant: Recessive: Phenotype: Genotype: Alleles: Homozygous: Heterozygous: Mendel's First Law: Mendel's Second Law: Haploid: Diploid: b) A yeast cell has the genotype AaBb, where the A and the B loci are on different chromosomes. Sketch the chromosome arrangement of this cell in meiosis I, when 2n = 4