28 ◆质粒 pSC101对大肠杆菌的转化技术突破 ● Another key characteristic of Cohen's plasmid was the ease of inserting it to a host cell. Cohen found that he could insert plasmids to fresh bacteria by suspending the latter in cold calcium chloride, then rapidly heating the bacteria to 42oC. So treated, the bacterial wall and plasma membrane open to permit the plasmids to pass through and into the cytoplasm. Cohen在重组DNA技术中杰出贡献28 ◆质粒 pSC101对大肠杆菌的转化技术突破 ● Another key characteristic of Cohen's plasmid was the ease of inserting it to a host cell. Cohen found that he could insert plasmids to fresh bacteria by suspending the latter in cold calcium chloride, then rapidly heating the bacteria to 42oC. So treated, the bacterial wall and plasma membrane open to permit the plasmids to pass through and into the cytoplasm. Cohen在重组DNA技术中杰出贡献