Weight matrix models ii 5'splice c signal GaY Gs G Background Con AG Pos Generic 2 5+6 A 0.25 A 03060.1 010.1 0.25 C 04010.0 0.102 G 0.25 G 020.208 0802 T 010101 0.00.5 T 0.25 S=S, S, SSg Odds ratio: R P(S|+)=P3S)P2S2)P1(S)P5(S)P6(S) P(S-)=Pbg(S1)Pb( S2)Pbg(S3)Pbg(S8)Pbg(Sg) Background model homogenous, assumes independenceWeight Matrix Models II 5’ splice signal C A G … G T Background Con: Pos -3 -2 -1 … +5 +6 A 0.3 0.6 0.1 … 0.1 0.1 C 0.4 0.1 0.0 … 0.1 0.2 G 0.2 0.2 0.8 … 0.8 0.2 T 0.1 0.1 0.1 … 0.0 0.5 Pos Generic A 0.25 C 0.25 G 0.25 T 0.25 S = S1 S2 S3 S4 S5 S6 S7 S8 S9 ( S1)P-2 ( S 2)P-1 ( S 3) ••• P 5 ( S 8)P 6 ( S 9 ) Odds Ratio: R = P(S|+) = P-3 P(S|-) = Pbg ( S1)Pbg ( S 2)Pbg ( S 3) ••• Pbg ( S 8)Pbg ( S 9) Background model homogenous, assumes independence