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Question 4 In E. coli, the fictitious AB operon is induced by the presence of Compound w. a diagram of the operon, its regulatory proteins and regulatory sites is shown below P gene a gene B PX promoter for the regulatory protein x gene for the regulatory protein of the AB operon P promoter for the aB genes sequence shown to be important for regulation by w structural gene for enzyme A structural gene for enzyme B The following table shows the genotypes of different E. coli strains with a wild-type AB operon and various mutant AB operons, and the number of molecules of protein a per cell in the absence or presence of Compound w(w or +w, respectively) a)In the table below, the symbol +indicates that the gene or control element is functional(wt)and indicates that the gene or control element is non-functional. Assume the genes not listed are wild Molecules of Strain E xpression Wild type 0200 inducible M1 200200 M2 0 200200 0 i For each strain on the table above, label the expression as either inducible, uninducible or constitutive ii)Based on the data shown above, does the regulatory protein X act as a repressor or an activator of the AB operon? Explain your reasoning7 Question 4 In E. coli, the fictitious AB operon is induced by the presence of Compound W. A diagram of the operon, its regulatory proteins and regulatory sites is shown below: gene X gene A P P S X gene B PX promoter for the regulatory protein X gene for the regulatory protein of the AB operon P promoter for the AB genes S sequence shown to be important for regulation by W A structural gene for enzyme A B structural gene for enzyme B The following table shows the genotypes of different E. coli strains with a wild-type AB operon and various mutant AB operons, and the number of molecules of protein A per cell in the absence or presence of Compound W (–W or +W, respectively). a) In the table below, the symbol "+" indicates that the gene or control element is functional (wt) and "–" indicates that the gene or control element is non-functional. Assume the genes not listed are wild type. Molecules of A Strain X P S A -W +W Expression Wild type + + + + 0 200 inducible M1 - + + + 200 200 M2 + - + + 0 0 M3 + + - + 200 200 M4 + + + - 0 0 i) For each strain on the table above, label the expression as either inducible, uninducible or constitutive. ii) Based on the data shown above, does the regulatory protein X act as a repressor or an activator of the AB operon? Explain your reasoning
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