pellet microsomes and ribosomes. 4.教学方法Teaching Methods: The teacher should recapitulate the key concept of the corresponding chapter taught in the theoretical class.The teacher should display the basic experimental steps to the students orally and remind students of the key points that determine the successful accomplishment of the experiment. 5.教学评价Evaluation: The teacher will evaluate the experimental procedure done by the students on-site.The students will finish the experimental report.The students should summarize the experimental result and attach necessary documents from their lab experiment.The students should also discuss the reason for experimental failures. The teacher will evaluate the report according to their experimental result and discussion. Experiment 8 Assaying of nucleic acid 1.教学目标Aims: (1) Comprehend the principle and method of mensuration of DNA and RNA. (2) Familiar with the principle and method of determination of DNA and RNA content. 2.教学重难点Key points: (1) Principle and method to extract DNA and RNA (2) Principle and method to quantify DNA and RNA 3.教学内容Content: There are two types of nucleic acid:DNA and RNA.DNA chiefly resides in cellular nucleus,and in cytoplasm RNA is most plentiful.In alkaline solution RNA cab be hydrolyzed,its ribose dehydrated to form furfurol,then furfurol can Kondens with orcinol to synthesize a green compound,the shade of green is in direct proportion to the amount of RNA.Heated in perchloric acid solution,DNA can be hydrolysed, its ribodesose dehydrated to form w-hydroxy-keto-pentanal,then it can react with diphenylamine to produce a blue compound,which chemical property is not clear,but the shade of blue is in direct proportion to the amount of DNA. 4.教学方法Teaching Methods:pellet microsomes and ribosomes. 4.教学方法 Teaching Methods: The teacher should recapitulate the key concept of the corresponding chapter taught in the theoretical class. The teacher should display the basic experimental steps to the students orally and remind students of the key points that determine the successful accomplishment of the experiment. 5.教学评价 Evaluation： The teacher will evaluate the experimental procedure done by the students on-site. The students will finish the experimental report. The students should summarize the experimental result and attach necessary documents from their lab experiment. The students should also discuss the reason for experimental failures. The teacher will evaluate the report according to their experimental result and discussion. Experiment 8 Assaying of nucleic acid 1.教学目标 Aims: （1） Comprehend the principle and method of mensuration of DNA and RNA. （2） Familiar with the principle and method of determination of DNA and RNA content. 2.教学重难点 Key points: （1） Principle and method to extract DNA and RNA （2） Principle and method to quantify DNA and RNA 3.教学内容 Content: There are two types of nucleic acid: DNA and RNA. DNA chiefly resides in cellular nucleus, and in cytoplasm RNA is most plentiful. In alkaline solution RNA cab be hydrolyzed, its ribose dehydrated to form furfurol, then furfurol can Kondens with orcinol to synthesize a green compound, the shade of green is in direct proportion to the amount of RNA. Heated in perchloric acid solution, DNA can be hydrolysed, its ribodesose dehydrated to form ω-hydroxy-keto-pentanal, then it can react with diphenylamine to produce a blue compound, which chemical property is not clear, but the shade of blue is in direct proportion to the amount of DNA. 4.教学方法 Teaching Methods: