2. regulation of acid-base balance 1)role of buffer HCO-r/cor 53%o Hb/HHb buffer system Hbo.2/HHbO2J 35% Pr/hPr 7 Phosphate 5 Henderson-hasselbalch ph= pKa+ lg Hco 3/H2CO31 =61+g20/1=61+13=74 buffer of fixed acid: hco3HCO buffer of volatile acid: hb/HHb2. regulation of acid-base balance 1) role of buffer HCO- 3 /H2CO3 53% Hb- /HHb buffer system HbO- 2 /HHbO2 35% Pr- /HPr 7% Phosphate 5% Henderson-Hasselbalch pH = pKa + lg [HCO- 3]/[H2CO3] = 6.1 + lg 20/1 = 6.1 + 1.3 =7.4 buffer of fixed acid: HCO- 3/H2CO3 buffer of volatile acid: Hb- /HHb