2. Electrons collected in nadh and FADH are released and transported to O, via the respiratory chain The chain is located on the convoluted inner D membrane(cristae) of mitochondria in eukaryotic cells(revealed by Eugene Kennedy and Albert Lehninger in 1948)or on the plasma membrane in prokaryotic cells A 1.14-volt potential difference(E )between NADH (-0.320 V)and O2(0.816 V)drives electron flow through the chain. D2. Electrons collected in NADH and FADH2 are released and transported to O2 via the respiratory chain • The chain is located on the convoluted inner membrane (cristae) of mitochondria in eukaryotic cells (revealed by Eugene Kennedy and Albert Lehninger in 1948) or on the plasma membrane in prokaryotic cells. • A 1.14-volt potential difference (E`0 ) between NADH (-0.320 V) and O2 (0.816 V) drives electron flow through the chain