Cell biology techniques Protoplast fusion(promote high frequencies of genetic recombinants) removing the cell wall with lytic enzymes in the presence of osmotic stabilizers. In the presence of fusogenic agent such as polyethylene glycol (eg), protoplasts are induced to fuse and form transient hybrids or diploids Regeneration of viable cells from the fused protoplasts.Cell biology techniques • Protoplast fusion (promote high frequencies of genetic recombinants) – removing the cell wall with lytic enzymes in the presence of osmotic stabilizers. – In the presence of fusogenic agent such as polyethylene glycol (PEG), protoplasts are induced to fuse and form transient hybrids or diploids. – Regeneration of viable cells from the fused protoplasts