诊断学理论与实践2005年第4卷第1期 化,为人类攻克癌症提供必要的实验研究材料。随 hematopoietic cell-specific tyrosine phosphatase SHP-1 着TMA技术的不断发展,病理学的作用将面临转 gene expression in natural killer cell lymphoma and va- 变,即在肿瘤分型时不再把重点放在组织学形态改 rious types of lymphomas/leukemias: combination anal 变上,而是从多方面、多水平地为临床治疗提供更 sis with cDNA expression array and tissue microarray [J 多资料。甚至在未来数年内,TMA技术的空间分辨 Am J Pathol,2001,1594):1495-1505 率将由现在的微米级向纳米级发展,相应的检测技 [12] Natkunam Y, Wamke RA, Montgomery K, et al. Analysis of MUMI/IRF4 protein expression using tissue microar- 术也将由现在的光学显微技术向共聚焦荧光显微 rays and immunohistochemistry [J]. Mod Pathol. 2001. 14 术、纳米显微术发展。相信在不久的将来,TMA技 7):686-694. 术在肿瘤的诊断和研究领域中将发挥更加重要的[13] Florell sr, Coffin cm, Holden jA,etal. Preservation of 作用。 RNA for functional genomic studies: a multidisciplinary tumor bank protocol[J). Mod Pathol, 2001, 14(2): 116-128. [参考文献] [14] Kipps TJ. Advances in classification and therapy of in- lolent B-cell malignancies[J]. Semin Oncol, 2002,29( [1 Kononen J, Bubendorf L Kallioniemi A et al. Tissue mi- croarays for high-throughput molecular profiling of tumor [15] Manley S, Mucci NR, De Marzo AM, et al.Relational specimens[J]. Nat Med, 1998, 4(7): 844-847 database structure to manage high-throughput tissue mi [21 Ekins R, Chu FW. Microarrays: their origins and applica croarray data and images for pathology studies focusing tions[J. Trends Biotechnol, 1999, 17(6: 217-218. on clinical outcome: the prostate specialized program of 3 Wooster R. Cancer classification with DNA microarrays research excellence model[J]. Am J Pathol, 2001, 1593) less more[J)? Trends Genet. 2000. 16(8): 327-329 837-843. [4 Moch H, Kononen T, Kallioniemi OP, et al. Tissue mi- [16 Bubendorf L, Kolmer M, Kononen J, et al. Hormone the- croarmays: what will they bring to molecular and anatomic rapy failure in human prostate: analysis by complementary pathology[J)? Adv Anat Pathol, 2001,8(1): 14-20 DNA and tissue microarrays[J). J Natl cancer Inst. 1999 Sallinen SL, Sallinen PK, Haapasalo HK, et al. Identifi- 1(20):1758-1764 catin of differentially expressed genes in human gliomas [17 Barlund M, Forozan F, Kononen J, et al. Detecting acti- by DNA microarray and tissue chip techniques[J. Cancer vation of ribosomal protein S6 kinase by complementary Res,2000.6023):6617-6622 DNA and tissue microarray analysis[J. J Natl Cancer Inst, 16 Richter J, Wagner U, Kononen J, et al. High-throughput 200092(15):1252-1259 tissue microarray analysis of cyclin e gene amplification [18 Parker RL, Huntsman DG, Lesack DW, et al. Assessment and overexpression in urinary bladder cancer [J]. Am J of interlaboratory variation in the immunohistochemical Pathol,2000157(3):787-794 determination of estrogen receptor status using a breast [7 Barlund M, Monni O, Kononen J, et al. Multiple genes at cancer tissue microarray [J). Am J Clin Pathol, 2002. 117 17q23 undergo amplification and overexpression in (5):723-728 breast cancer[J]. Cancer Res, 2000, 60(19): 5340-5344 [1】]王学民,李军,杜波,等.组织芯片的制备技术 8 Harris NL Jaffe ES, Diebold J, et al. The World Health 诊断病理学杂志,2002,96):370-371,01 Organization Classification of neoplastic diseases of the [20]周小鸽,张劲松,张小平,等.组织芯片技术在检测正 haematopoitic and lymphoid tissues: report of the Clinical 常组织和肿瘤组织抗原表达中的应用中华病理学 Advisory Committee Meeting, Airlie House, Virginia, 杂志,2002,31(2):181-182 November 1997[J). Histopathology, 2000,36(1): 69-86 [21]张建中,韩瑞刚,郑艳华,等.多肿瘤组织芯片在评价 19 Gaiser T, Thorns C, Merz H, et al. Gene profiling in 肿瘤细胞增殖与调亡状态研究中的作用J肿瘤防治 anaplastic large-cell lymphoma-derived cell lines with 杂志,2004,11(1)25-28 cDNA expression arrays(J). J Hematother Stem Cell Re.22)吕军,黄英武,向正华,等.组织芯片在筛选新型肿 200211(2:423-428 瘤分子标志物中的应用J生命的化学,2003,23(2:100 [10] Husson H. Carideo EG, Nerberg D, et al. Gene expres- sion profiling of follicular lymphoma and normal germinal23]石群立,孟奎,周晓军,等组织芯片检测乳腺癌相 enter B cells using cDNA arrays[J]. Blood, 2002,99(1) 关癌基因产物表达临床与实验病理学杂志,2003 282-289 [11 Oka T. 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