ruker 9 Two Bruker 400 MHz DPX-400 Spectrometers H NMR 核磁共振波谱分析法的特点及应用范围 ntegrate,Enlarge and Prin 的肉分桥用4 49 Fig.1. Schematic of the setup and probe design for CapNMR spectroscopy. CE=capillary electrophoresis. In CapNMR the sample has to be dissolved in only 5–10 μL of solvent. 50 51 Bruker 700 MHz Avance III Spectrometer with carbon-optimized cryoprobe This instrument is one of the most sensitive carbon￾detecting magnets in the world - capable of measuring a high quality carbon spectrum on 1000+ molecular weight samples as small as 0.1 mg. 52 Two Bruker 400 MHz DPX-400 Spectrometers 53 (1) NMR是化合物分子结构分析的最重要方法之一。尤其适 用于不能获得单晶的化合物或液体（包括溶液中）的化合物 的构型、构象的结构分析。 (2) 灵敏度比较低。一般要用mg以上的试样作测试，很少作 定性分析。定量分析精确度、准确度较差。 (3) 在化学反应动力学方面有独特的应用。可用于研究分子 内部基团的运动（内旋转），测定反应速度常数，也可以监 视一些化学反应的进行过程。 (4) NMR spectra are capable of supplying information about molecular interactions in solution. When a drug interacts with a receptor in a reversible manner, a number of effects may be observed in the spectra due to the exchange of the molecules between free and bound states. 核磁共振波谱分析法的特点及应用范围 54 Integrate, Enlarge and Print 1H NMR