BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Lecture 13: Molecular Devices Last time biological strategies for inorganic templating by organic materials Biomimetic organic template materials Biomimesis of bone Today Reading V Vogel, 'Reverse engineering: Learning from proteins how to enhance the performance of synthetic nanosystems, MRS Bull. Dec. 972-978(2002) Overview to date Current Road Map of the course: Started with degradable synthetic polymers-structural and controlled release materials Discussed modifying degradable materials for biological recognition Moved to controlled release devices fabricated from degradable polymers Next, hydrogel materials for drug delivery, tissue engineering and lab-on-a-chip applications o Structure, what are they made of o Theory of gel swelling for neutral and ionic gels Biomineralization: approaches used by biology and how we are trying to mimic them o Future materials for hard tissue engineering So far, largely looking at macroscopic materials MOk o Materials from which micron-sized or larger scaffolds, drug delivery devices and gels are fabricated Moving to smaller length scales: molecules and aggregates of molecules, we come to some new applications Performing molecular-level fund o Delivering molecular cargos to cells(labeling or treating cells) Application areas we'll focus on: Molecular devices o Length scale of one or a few molecules) o Single-molecule switches o Molecular motors Nano-to micro-scale drug carriers and detection reagents o(Length scale of supramolecular aggregates to many-molecule aggregates) Drug targeting Molecular Devices Current Approaches to Molecular Devices based on Protein-polymer h 3 examples we'll discuss 1. Use synthetic polymers to control 'on' and off state of a protein 2. Use engineered surfaces to direct the function of proteins 3. Use engineered proteins to build nano-motorized devices on surfaces Lecture 13-Hybrid macromolecules 1 of 13BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Lecture 13: Molecular Devices Last time: biological strategies for inorganic templating by organic materials Biomimetic organic template materials Biomimesis of bone Today: molecular devices Reading: V. Vogel, ‘Reverse engineering: Learning from proteins how to enhance the performance of synthetic nanosystems,’ MRS Bull. Dec. 972-978 (2002) Overview to date Current Road Map of the course: ƒ Started with degradable synthetic polymers – structural and controlled release materials ƒ Discussed modifying degradable materials for biological recognition ƒ Moved to controlled release devices fabricated from degradable polymers ƒ Next, hydrogel materials for drug delivery, tissue engineering, and lab-on-a-chip applications o Structure, what are they made of o Theory of gel swelling for neutral and ionic gels ƒ Biomineralization: approaches used by biology and how we are trying to mimic them o Future materials for hard tissue engineering ƒ So far, largely looking at ‘macroscopic’ materials o Materials from which micron-sized or larger scaffolds, drug delivery devices and gels are fabricated ƒ Moving to smaller length scales: molecules and aggregates of molecules, we come to some new applications o Performing molecular-level functions o Delivering molecular cargos to cells (labeling or treating cells) Application areas we’ll focus on: ƒ Molecular devices o (Length scale of one or a few molecules) o Single-molecule switches o Molecular motors ƒ Nano- to micro-scale drug carriers and detection reagents o (Length scale of supramolecular aggregates to many-molecule aggregates) ƒ Drug targeting Molecular Devices Current Approaches to Molecular Devices based on Protein-polymer hybrids ƒ 3 examples we’ll discuss: 1. Use synthetic polymers to control ‘on’ and ‘off state of a protein 2. Use engineered surfaces to direct the function of proteins 3. Use engineered proteins to build nano-motorized devices on surfaces Lecture 13 – Hybrid macromolecules 1 of 13