lacz Fusion Gene tagged by transposon can be cloned Segment of dNa adjacent to transposon Can be used as hybridization probe to clone wild type locus Can also be cloned by complementation Cloned dna can be sequenced BIOL 533 Lecture 4BIOL 533 8 Lecture 4 lacZ Fusion • Gene tagged by transposon can be cloned – Segment of DNA adjacent to transposon: • Can be used as hybridization probe to clone wild￾type locus • Can also be cloned by complementation • Cloned DNA can be sequenced