V. SDS-COOMASSIE STAINING AND DESTAINING: 0.25g Coomassie Blue R-250 was dissolved in 50ml methanoL added 8ml acetic acid and 42ml H20 to 100ml D 200ml methanol 70ml acetic acid. added H20 to 1000ml The gels are stained in staining solution for 1-2hours, and are then soaked in destaining solution for 1-2hours, it is necessary to change fresh destaining solution several times. VI GEL DRYING: The gels are first soaked in 30% Methanol/10%Glycerol solution for 30 minutes. It is carefully removed and sandwiched between a double layer of gel drying membrane on the drying frame(keep drying overnight)202 V. SDS-COOMASSIE STAINING AND DESTAINING: *Staining solution: 0.25g Coomassie Blue R-250 was dissolved in 50ml methanol, added 8ml acetic acid and 42ml H2O to 100ml. *Destaining solution: 200ml methanol, 70ml acetic acid, added H2O to 1000ml. The gels are stained in staining solution for 1~2hours, and are then soaked in destaining solution for 1~2hours, it is necessary to change fresh destaining solution several times. VI. GEL DRYING: The gels are first soaked in 30% Methanol/10%Glycerol solution for 30 minutes. It is carefully removed and sandwiched between a double layer of gel drying membrane on the drying frame(keep drying overnight)