5-capping The 5'end is modified by addition of m'G residue, called a cap. The is nucleotide is added to the new transcript in the reversed orientation giving 5-5 linkage. The sugars on the first and secondtranscribed nucleotides are methylated. m'gNmpNmp The transcripts are thus stabilized for preventing against 5'-exonuclease digestion. The cap is also important for splicing, nuclear transport and translationThe 5’ end is modified by addition of m7G residue, called a cap. The is nucleotide is added to the new transcript in the reversed orientation, giving 5’-5’ linkage. The sugars on the first and secondtranscribed nucleotides are methylated. m7GNmpNmp The transcripts are thus stabilized for preventing against 5’-exonuclease digestion. The cap is also important for splicing, nuclear transport and translation. 1 5’-capping :