Diagnostic Methods Seroconversion takes up to 3 months HIV antibodies detected by ELISA HIV antigens detected by Western blotting Plasma viral load is determined by PCR or nucleic acid hybridization • Seroconversion takes up to 3 months • HIV antibodies detected by ELISA • HIV antigens detected by Western blotting • Plasma viral load is determined by PCR or nucleic acid hybridization Diagnostic Methods