34监测蛋白质 Monitoring proteins 800 700 500 e400 300 100 0 0100200300400500600700800 c(SSB)/nM Figure 3. Continuous fluorescence titration of the molecular beacon with SSB. Each fluorescence value(.) at a certain SSB concentration is the average of ten readings of the fluorescence after the binding has reached uilibrium(corresponding to the plateau part of the time-scan curve shown in Figure 2). The dilution effect has been considered processing. The concentration of the MB was 250 nM. The stoichiometric ratio of MB to SsB was determined by the intersection point of two straight lines(-)extended, respectively, from the initial linear part and the plateau part of the titration curve(---). The results indicate that one SSB molecule binds to one mB molecule3.4 监测蛋白质(Monitoring proteins)