30 ◆Cohen与Boyer合作创建重组DNA分子 ●Cohen had been conducting fruitful experiments with plasmids, but he was experiencing difficulty cutting open the plasmids. ●Boyer's EcoRI enzyme seemed to be the ideal solution, so Cohen suggested they join forces. ●They would use his plasmids and Boyer's enzyme to recombine the plasmid DNA. ■ First they would try to recombine two plasmids to form a single plasmid. ■ If successful, they would attempt to bring DNA from a foreign species into the plasmid to produce a recombinant DNA molecule. Boyer agreed, and the bargain was struck. Cohen在重组DNA技术中杰出贡献30 ◆Cohen与Boyer合作创建重组DNA分子 ●Cohen had been conducting fruitful experiments with plasmids, but he was experiencing difficulty cutting open the plasmids. ●Boyer's EcoRI enzyme seemed to be the ideal solution, so Cohen suggested they join forces. ●They would use his plasmids and Boyer's enzyme to recombine the plasmid DNA. ■ First they would try to recombine two plasmids to form a single plasmid. ■ If successful, they would attempt to bring DNA from a foreign species into the plasmid to produce a recombinant DNA molecule. Boyer agreed, and the bargain was struck. Cohen在重组DNA技术中杰出贡献