Figure 5.15 Two methods of a viable count(plate count/colony count):spread- plate method and pour-plate method Spread-plate method Surface colonies Incubation Sample is pipetted onto Sample is spread evenly Typical spread-plate surface of agar plate over surface of agar results (0.1 ml or less) using sterile glass spreader Pour-plate method Surface colonies Solidification Subsurface Bun可Bu and incubation colonies Sample is pipetted into Sterile medium is Typical pour-plate sterile plate added and mixed results well with inoculum 活菌记数的两种平板记数法：涂布法与混菌法Figure 5.15 Sample is pipetted onto surface of agar plate (0.1 ml or less) Sample is spread evenly over surface of agar using sterile glass spreader Typical spread-plate results Surface colonies Surface colonies Subsurface colonies Typical pour-plate results Sample is pipetted into sterile plate Sterile medium is added and mixed well with inoculum Spread-plate method Pour-plate method Incubation Solidification and incubation Two methods of a viable count (plate count/colony count): spread￾plate method and pour-plate method 活菌记数的两种平板记数法: 涂布法与混菌法