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112 J.Teissie et al.Bioelectrochemistry 55 (2002)107-112 the molecular processes affecting the cell organization [15]V.Ganeva,B.Galutzov,J.Teissie,Electric field mediated loading of during the field pulses. macromolecules in intact yeast cells is critically controlled at the wall level,Biochim.Biophys.Acta 1240(1995)229-236. [16]V.Ganeva,B.Galutzov,N.Eynard,J.Teissie,Electroinduced extrac- tion of B-galactosidase from Kluyveromyces lactis,Appl.Microbiol. Acknowledgements Biotechnol.56(2001)411-413. [17]French patent 0013415. Financial supports from EDF and NATO are acknowl- [18]M.C.Vernhes,These d'Universite,Universite Toulouse 3.1999. edged. [19]D.Knorr,Novel approaches in food processing technology:new tech- nologies for preserving foods and modifying function,Cur.Opin. Biotechnol.10(1999)485-491. [20]C.H.King,E.B.Shotts,R.E.Wooley,K.G.Porter,Survival of coli- References forms and bacterial pathogens within protozoa during chlorination, Appl.Environ.Microbiol.54 (1998)3023-3033. 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Conte, Electrofusion of large volumes of cells in culture: 2, Cells growing in suspension, Bioelectrochem. Bioenerg. 19 (1988) 59 – 66. [12] M.P. Rols, D. Coulet, J. Teissie´, Highly efficient transfection of mam￾malian cells by electric field pulses: application to large volumes of cell culture by using a flow system, Eur. J. Biochem. 206 (1992) 115 – 121. [13] S. Sixou, J. Teissie´, Specific electropermeabilization of leucocytes in a blood sample and application to large volumes of cells, Biochim. Biophys. Acta 1028 (1990) 154 – 160. [14] E. Meilhoc, J. Teissie´, J.M. Masson, High efficiency transformation of intact yeast cells by electric field pulses, Bio/Technology 8 (1990) 223 – 227. [15] V. Ganeva, B. Galutzov, J. Teissie´, Electric field mediated loading of macromolecules in intact yeast cells is critically controlled at the wall level, Biochim. Biophys. Acta 1240 (1995) 229 – 236. [16] V. Ganeva, B. Galutzov, N. Eynard, J. 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