Example: Screen of the AD-array with rPc19 as a bait This is the activation domain array consisting of 6144 yeast colonies each expressing a different fusion of the gal4 activation domain and one of the yeast ORFs The array is mated to the rPC19 bait and diploids are pinned to selective plates This is a set of selective plates (-- His-Ade)on which only colonies grow that contain RPC19-interacting proteins Stan Field s web site http://depts.washingtonedu/sfields/images/rpc19.html Courtesy of stanley Fields. Used with permissionStan Field’s web site http://depts.washington.edu/sfields/images/RPC19.html Courtesy of Stanley Fields. Used with permission