3capacitation and acrosome reaction of spermatozoa: R5 capacitation factor; secreted by epi. cells of oviduct, capacity of releasing acrosome enzymes 4)quality and quantity of spermatozoa 2-6ml,100,000,000/m, <1.5 ml; or <10,000,000 abnormal sperm >30%;or capacity for mobile< 70% 5)meeting of sperm and ovum within 24 hrs3) capacitation and acrosome reaction of spermatozoa: R5 • capacitation factor: secreted by epi. cells of oviduct, capacity of releasing acrosome enzymes 4) quality and quantity of spermatozoa: • 2-6 ml, 100,000,000/ml, • <1.5 ml; or <10,000,000; abnormal sperm >30%; or capacity for mobile< 70% 5) meeting of sperm and ovum within 24 hrs