5. The conversion of glucose 6-phosphate to glucose is the last bypassing step The reaction is catalyzed by glucose 6-phosphatase instead of hexokinase). The enzyme is present on the lumen side of the ER membrane of hepatocytes and renal cells The enzyme is not present in muscle or brain cells, where gluconeogenesis does not occur coe6 phosphate+H2O—→ glucose+P1△G=-13.8 kJ/mol5. The conversion of glucose 6-phosphate to glucose is the last bypassing step • The reaction is catalyzed by glucose 6-phosphatase (instead of hexokiase). • The enzyme is present on the lumen side of the ER membrane of hepatocytes and renal cells. • The enzyme is not present in muscle or brain cells,where gluconeogenesis does not occur