ho lcus ot链6 Eag I(166) Sal I(79) EcoR K192) he l( T7 promoter prmer #69348-3 pET upstream prmer #69214-3 Ndel Nhe I T.TE TATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGGCTAGCATGACTGGTGGACAGC MetGlyser SerHISHISHISHISHIsHIsSerSerGIyL BamH I EcoR I Sac I Sall. Hind. IlL. AIGGGTCGCGGATCO AGCACCACCACCACCACCACTGAGATCCGGCTGC TAACAAAGCCC pET-28a(. etGlyAroGlySer GluPheGluLeuArgArgG Thr ArgAlcProProProProProl euArgSerGlycysEnd GGTCGGGATCCGAATTCGAGCTCCGTCGACAAGCT TGCGGCCGCAC TCGAGCACCACCACCACCACCAC TGAGATCCGGCTGCTAACAAAGCCC PET-28b(-) GlyArgAspProAsnSer Ser Ser VolAsplysLeuAloAlaAloLeuGI uHISHISHI SHISHISHISEno CGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGC AC TCGAGCACCACCACCACCACCACTGAGA CTGCTAACAAAGCCC pET-28c(“ Arol leArgl leArgAlaProSerThr SerLeuArgProH sSer SerThrThr ThrThrThrTnrGlul TheLysp Bpu1102 7 terminator GAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGECTCTAAACGGGTCTTGAGGGGTTTTTTG T7 terminator primer #69337- pET-28a-c(+) cloning/expression region SssS I(3397 PshA I(1968 BspLU11 13224 ap310 sstt071(23 Tth1111(299)