Rich Media Are Required for Culture of animal cells o Nine amino acids referred to as the essential amino acids, cannot be synthesized by adult vertebrate animals and thus must be obtained from their diet animal cells grown in culture also must be supplied with these nine amino acids namely histidine, isoleucine leucine lysine valine addition, most cultured cells require cysteine, glutamine, and tyrosine o The other essential components of a medium for culturing animal cells are vitamins which the cell cannot make at all or in adequate amounts various salts glucose; and serum, the noncellular part of the bloodRich Media Are Required for Culture of Animal Cells • Nine amino acids, referred to as the essential amino acids, cannot be synthesized by adult vertebrate animals and thus must be obtained from their diet. Animal cells grown in culture also must be supplied with these nine amino acids, namely, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine. • addition, most cultured cells require cysteine, glutamine, and tyrosine. • The other essential components of a medium for culturing animal cells are vitamins, which the cells cannot make at all or in adequate amounts; various salts; glucose; and serum, the noncellular part of the blood