ON BOARD o Length scales in cell adhesion o Size of integrins, focal contacts, relationship to cell size, fiber spacing DRAW ON BOARD focal contacts"(SLIDEl 12-15nm 12-15nm Actin stress fibers LXSN LV3SN Interference reflection microscopy lark spots indicate cell-substrate eparations 50 nm 20 different integrins known, many different pairs possible with different ligand specificities, thus cell-specific adhesion can be modulated by ECM Lecture 3- Biological Recognition 5of15Ca++ Ca++ Ca++ Ca++ Ca++ Ca ++ Ca+ Ca++ ON BOARD: o Length scales in cell adhesion: o Size of integrins, focal contacts, relationship to cell size, fiber spacing DRAW ON BOARD Integrin structure (Lodish) focal contacts2 (SLIDE) Actin stress fibers Interference reflection microscopy: dark spots indicate cell-substrate separations < 50 nm (stress fibers pics from Maheshwari et al.) • 20 different integrins known, many different pairs possible with different ligand specificities, thus cell-specific adhesion can be modulated by ECM Lecture 3 – Biological Recognition 5 of 15