第6期 林海等：降解造纸废水木质素菌种的筛选鉴定及应用 .573 长所需足够的氧，大大提高了其适应环境的能力，在 参考文献 培养初期即表现出良好的降解效果， [1]庾晋，周洁.我国造纸业发展现状和趋势.黑龙江造纸，2001， 4.10 3结论 [2]林云琴，周少奇.白腐菌降解纤维素和木质素的研究进展.环 (1)通过采样、驯化、分离和鉴定等一系列实 境技术，2003,4：29 [3]韦朝海，任源.淮河流域酿造业生产与环境污染的损益分析， 验，培养出能够降解木质素的生物降解菌一白 食品与发酵工业，1998,24(5).64 腐菌， [4]Michrael G.Gold M H.Molecular biology of thre lignin-degrad- (2)采样的原水为河南省淇县造纸厂造纸废 ing basidiomycete Phranerochraete chrrysosporium.Microbiol 水，驯化用污泥为高碑店污水处理厂二沉池回流污 Rew,1993,57(3):605 泥，待原水比例提至100%降解2d后出水C0Dc,去 [5]Glenn J K.Gold M H.Decolorization of several polymeric dyes by 除率达到74%. the lignin-degrading basidiomycete Phranerochroete chrrysospori- m.Appl Environ Microbiol,1983.45:1741 (③)采用稀释倒平板法从驯化后的污泥中分离 [6]Crawford R L.Liglin Biodegration and Transformation.New 出三种菌，经平板划线法纯化得到三种纯菌，通过 York:John Wiley &Sons Inc.1981:42 白腐菌的特征反应一Bavendamm反应一证实 [7]孙先锋，张志杰，崔红军·造纸黑液木质素降解微生物的分离 菌落为白腐菌，对白腐菌进行革兰氏染色实验，判 和降解特性研究，环境工程，2002,20(3)：78 [8]林刚，文湘华，钱易，应用白腐真菌技术处理难降解有机物的 定该菌种为革兰氏阳性菌，菌种形态为短杆状，菌 研究进展.环境污染治理技术与设备，2001,2(4)：1 种生长周期为70h,并初步判定其为好氧型的产 [9]钱存柔，黄仪秀微生物学实验教程.北京：北京大学出版社， 碱菌， 1999,58 (4)在原水比例70%、pH初始值为5以及初始 [10]钱大益，朱益丹，宋存义，等.光合细菌的生长条件.北京科 溶解氧浓度为4mgL条件下，最终CODc去除率 技大学学报，2005,27(2)：156 为80.9%. Screening and identification of strain degrading lignin and its application in paper- making wastew ater treatment LIN Hai,LU Gang,ZHA NG Qingna,LV Lvzhou,JIA Xiuming Civil and Environmental Engineering School,University of Science and Technology Beijing.Beijing 100083.China ABSTRACI To improve the biological treatment effect of paper making wastewater,the screening and identi- fication of strain degrading lignin and its application were carried out.The strain able to degrading lignin was obtained after a series of experiments including sampling,domesticating,separating and identifying etc,and it was defined as a white rot fungi,short rod,aerobic alkali producing bacteria based on investigations on its phys- iological and biochemical prosperities.After Gram s stain,the strain was identified as a Gram positive bacteri- um.In further research,the strain strongly capable of degrading was selected to apply in wastewater treatment experiment and the results showed that this bacterium possessed a removal rate of 80.9for CODCr in wastew- ater from paper mills,which displayed good prospects of application. KEY WORDS wastew ater treatment;paper making wastewater;lignin;white-rot fungi;screening;domesti- cation长所需足够的氧‚大大提高了其适应环境的能力‚在 培养初期即表现出良好的降解效果． 3 结论 （1） 通过采样、驯化、分离和鉴定等一系列实 验‚培养出能够降解木质素的生物降解菌－－－白 腐菌． （2） 采样的原水为河南省淇县造纸厂造纸废 水‚驯化用污泥为高碑店污水处理厂二沉池回流污 泥‚待原水比例提至100％降解2d 后出水 CODCr去 除率达到74％． （3） 采用稀释倒平板法从驯化后的污泥中分离 出三种菌‚经平板划线法纯化得到三种纯菌．通过 白腐菌的特征反应－－－Bavendamm 反应－－－证实 菌落为白腐菌．对白腐菌进行革兰氏染色实验‚判 定该菌种为革兰氏阳性菌‚菌种形态为短杆状．菌 种生长周期为70h‚并初步判定其为好氧型的产 碱菌． （4） 在原水比例70％、pH 初始值为5以及初始 溶解氧浓度为4mg·L －1条件下‚最终 CODCr去除率 为80∙9％． 参 考 文 献 ［1］ 庾晋‚周洁．我国造纸业发展现状和趋势．黑龙江造纸‚2001‚ 4：10 ［2］ 林云琴‚周少奇．白腐菌降解纤维素和木质素的研究进展．环 境技术‚2003‚4：29 ［3］ 韦朝海‚任源．淮河流域酿造业生产与环境污染的损益分析． 食品与发酵工业‚1998‚24（5）：64 ［4］ Michrael G‚Gold M H．Molecular biology of thre lignin-degrad￾ing basidiomycete Phranerochraete chrrysosporium． Microbiol Rev‚1993‚57（3）：605 ［5］ Glenn J K‚Gold M H．Decolorization of several polymeric dyes by the lignin-degrading basidiomycete Phranerochraete chrrysospori￾um．Appl Environ Microbiol‚1983‚45：1741 ［6］ Crawford R L．Liglin Biodegration and Transformation．New York：John Wiley ＆ Sons Inc‚1981：42 ［7］ 孙先锋‚张志杰‚崔红军．造纸黑液木质素降解微生物的分离 和降解特性研究．环境工程‚2002‚20（3）：78 ［8］ 林刚‚文湘华‚钱易．应用白腐真菌技术处理难降解有机物的 研究进展．环境污染治理技术与设备‚2001‚2（4）：1 ［9］ 钱存柔‚黄仪秀．微生物学实验教程．北京：北京大学出版社‚ 1999：58 ［10］ 钱大益‚朱益丹‚宋存义‚等．光合细菌的生长条件．北京科 技大学学报‚2005‚27（2）：156 Screening and identification of strain degrading lignin and its application in paper￾making wastewater treatment LIN Hai‚LU Gang‚ZHA NG Qingna‚LV Lvz hou‚JIA Xiuming Civil and Environmental Engineering School‚University of Science and Technology Beijing‚Beijing100083‚China ABSTRACT To improve the biological treatment effect of paper-making wastewater‚the screening and identi￾fication of strain degrading lignin and its application were carried out．The strain able to degrading lignin was obtained after a series of experiments including sampling‚domesticating‚separating and identifying etc‚and it was defined as a white-rot fungi‚short-rod‚aerobic alkal-i producing bacteria based on investigations on its phys￾iological and biochemical prosperities．After Gram’s stain‚the strain was identified as a Gram-positive bacteri￾um．In further research‚the strain strongly capable of degrading was selected to apply in wastewater treatment experiment and the results showed that this bacterium possessed a removal rate of80∙9％ for CODCr in wastew￾ater from paper mills‚which displayed good prospects of application． KEY WORDS wastewater treatment；paper-making wastewater；lignin；white-rot fungi；screening；domesti￾cation 第6期 林 海等： 降解造纸废水木质素菌种的筛选鉴定及应用 ·573·