Commercial Sterilization to Destroy C.botulinum Endospores 12D treatment kills 1012 endospores Surviving endospores of thermophilic anaerobes cause spoilage with gas Or flat-sour spoilage • 12D treatment kills 1012 endospores • Surviving endospores of thermophilic anaerobes cause spoilage with gas • Or flat-sour spoilage Commercial Sterilization to Destroy C. botulinum Endospores