1301 pathogenic gene mutation in human embryos. Nature, 2017 20 Ye H, Xie M, Xue S, et al. Self-adjusting synthetic gene circuit 48(7668):413-419 for correcting insulin resistance. Natute Biomedical Engineering, 26 Liu Z, Cai Y, Wang Y, et al. Cloning of macaque monkeys by 2017,1(1):0005 somatic cell nuclear transfer. Cell, 2018, 174(1): 245 21 Shao J, Xue S, Yu G, et al. Smartphone-controlled optogenetically 27 Feng C, Wang X, Shi H, et al. Generation of apoe deficient dogs engineered cells enable semiautomatic glucose homeostasis in via combination of embryo injection of crispr/cas with somatic diabetic mice. Science Translational Medicine, 2017, 9(387) cell nuclear transfer. Journal of Genetics and Genomics 2018. 4 eaal2298 (1):47- 22 Xue S, Yin J, Shao J, et al. A synthetic-biology-inspired therapeut 28 Yan S, Tu Z, Liu Z, et al. A huntingtin knockin pig model strategy for targeting and treating hepatogenous diabete recapitulates features of selective neurodegeneration in Molecular Therapy, 2017, 25(2): 443-455 huntingtons disease Cell, 2018, 173(4): 989-1002 23 Liang P, Xu Y, Zhang X, et al. CRISPR/Cas9-mediated gene 29 Ruella M, Xu J, Barrett D M, et al. Induction of resistance to zygotes. Protein and Cells, 2015 chimeric antigen receptor T cell therapy by transduction of a single 6(5):363-372. leukemic B cell Nature Medicine, 2018, 24(10): 1499-1503 24 Kang X Huang Y, et al. Introducing precise genetic 30 US Department of Health and Human Services. Early Clinical Trials modifications into human 3PN embryos by CRISPR/Cas-mediated ith Live Biotherapeutic Products: Chemistry, Manufacturing, and genome editing. Journal of Assisted Reproduction and Genetics, ControlInformation.[2016-06-01].https://www.fda.gov/downloads/ 2016,33(5):581-588 Biologics Blood Vaccines/Guidance Compliance Regulatorylnformati 25 Ma H. Marti-GutierrezN. Park Sw. et al. Correction of a on/ Guidances/General/UCM292704. pdf. Progress of Synthetic Biology Research in Medical Applications CUI Jinming WANG Liwei CHANG Zhiguang ZANG Zhongsheng LIU Chenli 1 Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China, 2 Bureau of Frontier Sciences and Education, Chinese Academy of Sciences, Beijing 100864, China Abstract In the field of medical applications, synthetic biology researchers design genetic circuits to modify human cells, or to modify synthetic organisms such as bacteria and viruses and make them interact with the human body. These artificially designed organisms are apable of perceiving disease-specific signals or artificial signals, targeting abnormal cells and foci, expressing reporter molecules or releasing therapeutic drugs, thus enabling the monitoring of human physiological conditions and the diagnosis and treatment of typical diseases such as tumors, metabolic diseases, and drug-resistant bacteria infections. This article will comprehensively describe some recent research progress Keywords synthetic cell, synthetic bacteria, synthetic virus, synthetic phage *Corresponding author 12262018年·第33卷·第11期1226 2018 年 . 第 33 卷 . 第 11 期 应用领域 1301. 20 Ye H, Xie M, Xue S, et al. Self-adjusting synthetic gene circuit for correcting insulin resistance. Natute Biomedical Engineering, 2017, 1(1): 0005. 21 Shao J, Xue S, Yu G, et al. Smartphone-controlled optogenetically engineered cells enable semiautomatic glucose homeostasis in diabetic mice. Science Translational Medicine, 2017, 9(387): eaal2298. 22 Xue S, Yin J, Shao J, et al. A synthetic-biology-inspired therapeutic strategy for targeting and treating hepatogenous diabetes. Molecular Therapy, 2017, 25(2): 443-455. 23 Liang P, Xu Y, Zhang X, et al. CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Protein and Cells, 2015, 6(5): 363-372. 24 Kang X, He W, Huang Y, et al. Introducing precise genetic modifications into human 3PN embryos by CRISPR/Cas-mediated genome editing. Journal of Assisted Reproduction and Genetics, 2016, 33(5): 581-588. 25 Ma H, Marti-Gutierrez N, Park SW, et al. Correction of a pathogenic gene mutation in human embryos. Nature, 2017, 548(7668): 413-419. 26 Liu Z, Cai Y, Wang Y, et al. Cloning of macaque monkeys by somatic cell nuclear transfer. Cell, 2018, 174(1): 245. 27 Feng C, Wang X, Shi H, et al. Generation of apoe deficient dogs via combination of embryo injection of crispr/cas9 with somatic cell nuclear transfer. Journal of Genetics and Genomics, 2018, 45 (1): 47-50. 28 Yan S, Tu Z, Liu Z, et al. A huntingtin knockin pig model recapitulates features of selective neurodegeneration in huntington’s disease. Cell, 2018, 173(4): 989-1002. 29 Ruella M, Xu J, Barrett D M, et al. Induction of resistance to chimeric antigen receptor T cell therapy by transduction of a single leukemic B cell. Nature Medicine, 2018, 24(10): 1499-1503. 30 US Department of Health and Human Services. Early Clinical Trials with Live Biotherapeutic Products: Chemistry, Manufacturing, and Control Information. [2016-06-01]. https://www.fda.gov/downloads/ BiologicsBloodVaccines/GuidanceComplianceRegulatoryInformati on/Guidances/General/UCM292704.pdf. Progress of Synthetic Biology Research in Medical Applications CUI Jinming1 WANG Liwei2 CHANG Zhiguang1 ZANG Zhongsheng1 LIU Chenli1* （1 Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; 2 Bureau of Frontier Sciences and Education, Chinese Academy of Sciences, Beijing 100864, China） Abstract In the field of medical applications, synthetic biology researchers design genetic circuits to modify human cells, or to modify synthetic organisms such as bacteria and viruses and make them interact with the human body. These artificially designed organisms are capable of perceiving disease-specific signals or artificial signals, targeting abnormal cells and foci, expressing reporter molecules or releasing therapeutic drugs, thus enabling the monitoring of human physiological conditions and the diagnosis and treatment of typical diseases such as tumors, metabolic diseases, and drug-resistant bacteria infections. This article will comprehensively describe some recent research progress. Keywords synthetic cell, synthetic bacteria, synthetic virus, synthetic phage *Corresponding author