.24. 北京科技大学学报 第29卷 redox potential,SO concentration and ferrous oxidation rate with time in bio culture as well as in bioleaching of chalcopyrite concentrate were elucidated.The bioleaching experiments show that using the trained sulfolobus strains at 75C in the shake blasting when the slurry mass fraction is below 10%,the chalcopyrite concentrate leaching rate could up to 90%after 150 h leaching.But when slurry mass fraction is up to 15%.after 350h leaching,the leaching rate is only 80%.It demonstrates that the slurry mass fraction has apparently effect on the leaching results. KEY WORDS biohydrometallurgy;chalcopyrite concentrate;bioleaching:thermophilic microorganism; solfolobus (上接第4页) Dynamical destabilization analysis of steep and heavy thick coal seam in a deep mine under the complex variable environment based on AE ZHANG Lijie),CAI Meifeng,LAI Xingping23),ZHANG Yong23),ZHA NG Haiyan2),LV Zhao hai23),REN Fenhua) 1)Civil and Environmental Engineering School,University of Science and Technology Beijing.Beijing 100083.China 2)Xi'an University of Science and Technology.Xi'an 710054.China 3)Key Lab for Western Safe Mining &Hacard Control.the Ministry of Education of China.Xi'an 710054.China ABSTRACT The No.5 coal of Huating Coal Mine in China is a deep and heavy thick coal seam.In the coal seam,the dependence of excavation and disturbance is very serious,the difference between the maximum and minimum principal stress levels is greater,and there are frequent destabilizations leading to hazards during min- ing.Based on the comparison of the acoustic emission(AE)experiment of coal-rock unstable crack propagation and destabilization with their in situ measurement,this paper investigated the regularity of various dynamics load and comprehensively analyzed the quantificational destabilization characters including variable dynamical specific energy of tension,resistance to compression,and proportional limit of shear under the complex variable excavation and geological environment. KEY WORDS heavy thick coal seam;acoustic emission (AE);complex variable environment;large scale mined-out area;dynamical destabilizationredox potential‚SO 2— 4 concentration and ferrous oxidation rate with time in bio culture as well as in bioleaching of chalcopyrite concentrate were elucidated．The bioleaching experiments show that using the trained sulfolobus strains at 75℃ in the shake blasting when the slurry mass fraction is below 10％‚the chalcopyrite concentrate leaching rate could up to 90％ after 150h leaching．But when slurry mass fraction is up to 15％‚after 350h leaching‚the leaching rate is only 80％．It demonstrates that the slurry mass fraction has apparently effect on the leaching results． KEY WORDS biohydrometallurgy；chalcopyrite concentrate；bioleaching；thermophilic microorganism； solfolobus （上接第4页） Dynamical destabilization analysis of steep and heavy thick coal seam in a deep-mine under the complex-variable environment based on AE ZHA NG L ijie 1‚2）‚CAI Meifeng 1）‚LAI Xingping 2‚3）‚ZHA NG Yong 2‚3）‚ZHA NG Haiyan 2）‚LV Zhao￾hai 2‚3）‚REN Fenhua 1） 1） Civil and Environmental Engineering School‚University of Science and Technology Beijing‚Beijing100083‚China 2） Xi’an University of Science and Technology‚Xi’an710054‚China 3） Key Lab for Western Safe Mining ＆ Hazard Control‚the Ministry of Education of China‚Xi’an710054‚China ABSTRACT The No．5coal of Huating Coal Mine in China is a deep and heavy thick coal seam．In the coal seam‚the dependence of excavation and disturbance is very serious‚the difference between the maximum and minimum principal stress levels is greater‚and there are frequent destabilizations leading to hazards during min￾ing．Based on the comparison of the acoustic emission （AE） experiment of coa-l rock unstable crack propagation and destabilization with their in-situ measurement‚this paper investigated the regularity of various dynamics load and comprehensively analyzed the quantificational destabilization characters including variable dynamical specific energy of tension‚resistance to compression‚and proportional limit of shear under the complex-variable excavation and geological environment． KEY WORDS heavy thick coal seam；acoustic emission （AE）；complex-variable environment；large scale mined-out area；dynamical destabilization ·24· 北 京 科 技 大 学 学 报 第29卷