Cell Culture/Organ Culture e Difficult Cell-lines often tumor lines that are genetically and physiologically different (immortal--many mutations) Removed from effects of other organs hormones · Cells grown in artificial media differ from∥Wivo Cell lines may not express same Ag on surface as When in animal BIOL 533 Lecture threeBIOL 533 5 Lecture Three Cell Culture/Organ Culture • Difficult • Cell-lines often tumor lines that are genetically and physiologically different (immortal—many mutations) • Removed from effects of other organs, hormones • Cells grown in artificial media differ from in vivo • Cell lines may not express same Ag on surface as when in animal