Isolation of fungal pathogens from infected plant tsst lesion placed in IO% clorox used to transfer with sterile popr otted Sections are Infected plant Sections from margin of Sterile forceps Tissue sections bl placed on for different durations sections towel to remove nutrient clorox excess medium in petri dish /签留 g→ Sections placed on In correct immersion A pure culfure of the pathogen is abtained by nutient media in le g 90)only the subculturing a segment of the pathogen growth order of immersion pc pathogen survives in in the previous plate into a new plate with ruir e medium time in clorox center of section and gr ows out of the tissue