Restriction enzyme an enzyme that recognizes and makes double-stranded breaks at specific DNa sequences Shotgun cloning making a gene library by closing random DNA fragments Site-directed mutagenesis a technique whereby a gene with a specific mutation can be constructed in vitro Synthetic dna a dna molecule made by a chemical process in a laboratory Transduction transfer of host genes from one cell to another by a virus Transformation transfer of bacterial genes involving free DNA Transposon a type of transposable element that carries genes in addition to those involved in transpositionRestriction enzyme an enzyme that recognizes and makes double-stranded breaks at specific DNA sequences Shotgun cloning making a gene library by closing random DNA fragments Site-directed mutagenesis a technique whereby a gene with a specific mutation can be constructed in vitro Synthetic DNA a DNA molecule made by a chemical process in a laboratory Transduction transfer of host genes from one cell to another by a virus Transformation transfer of bacterial genes involving free DNA Transposon a type of transposable element that carries genes in addition to those involved in transposition