For each electron to be transferred from dinitrogenase reductase to dinitrogenase two ATPs are hydrolyzed causing a conformational change which reduces the electron affinity for the reductase (i.e., an increased reducing power) The oxidized and reduced dinitrogenase reductase dissociates from and associates with the dinitrogenase, respectively The nitrogenase is in imperfect enzyme: H, is formed along with NH• For each electron to be transferred from dinitrogenase reductase to dinitrogenase, two ATPs are hydrolyzed causing a conformational change which reduces the electron affinity for the reductase (i.e., an increased reducing power). • The oxidized and reduced dinitrogenase reductase dissociates from and associates with the dinitrogenase, respectively. • The nitrogenase is in imperfect enzyme: H2 is formed along with NH3