Darkfield Illumination Eye Eye Ocular lens Objective lens Only light reflected by the specimen is 。Light objects are captured by the Specimen objective lens visible against a Unreflected light dark background. Condenser lens Opaque disc 。Light reflected off Light Light the specimen enters the objective lens. (a)Brightfield (b)Darkfield Figure 3.4a,b• Light objects are visible against a dark background. • Light reflected off the specimen enters the objective lens. Darkfield Illumination Figure 3.4a, b