Leading and Lagging Strands Leading strand: DNa synthesis occurs continuously from the 5 -phosphate to the 3-hydroxyl Lagging strand: DNA synthesis occurs discontinuously Enzymes in lagging strand synthesis DNA primase, DNA polymerase III and I, DNA ligaseLeading and Lagging Strands Leading strand: DNA synthesis occurs continuously from the 5’-phosphate to the 3’-hydroxyl; Lagging strand: DNA synthesis occurs discontinuously. Enzymes in lagging strand synthesis: DNA primase, DNA polymerase III and I, DNA ligase