Question 3 The primer shown below is used to sequence the following template dNA template dna 5 -ACTGAC-3' 5 -ACCACTAACGTCAGT-3' Draw the resulting DNA fragments that would be produced from each of the 4 sequencing reactions at the correct position (length in nucleotides) as they would appear on the diagram of the sequencing gel DNA length G T C (nts) 111111 54321098765432 B Polio has been practically eliminated from the American population, however, in countries have little or no access to vaccinations, it is still prevalent. As a biologist with a global seek to create a transgenic banana that produces the protein used in the vaccine against consuming these bananas, individuals will develop immunity against the disease. The gene for this protein has already been cloned into a plasmid with a kanamycin-resistance gene(pkR-polio). you need to attach to the gene a banana-specific promoter and dna sequences that will allow the gene to be incorporated into banana DNA. These sequences are contained in the pBan plasmid, which carries a gene for ampicillin resistance. Maps of these two plasmids are shown on the next page, including mportant restriction sites and distances (in base pairs) between the sites4 Question 3 A. The primer shown below is used to sequence the following template DNA. primer: template DNA: 5'-ACTGAC-3' 5'-ACCACTAACGTCAGT-3' Draw the resulting DNA fragments that would be produced from each of the 4 sequencing reactions at the correct position (length in nucleotides) as they would appear on the diagram of the sequencing gel below. G A T C + DNA length (nts) 15- 14- 13- 12- 11- 10- 9- 8- 7- 6- 5- 4- 3- 2- 1- B. Polio has been practically eliminated from the American population, however, in countries where people have little or no access to vaccinations, it is still prevalent. As a biologist with a global vision, you seek to create a transgenic banana that produces the protein used in the vaccine against polio. By consuming these bananas, individuals will develop immunity against the disease. The gene for this protein has already been cloned into a plasmid with a kanamycin-resistance gene (pKR-polio). You need to attach to the gene a banana-specific promoter and DNA sequences that will allow the gene to be incorporated into banana DNA. These sequences are contained in the pBAN plasmid, which carries a gene for ampicillin resistance. Maps of these two plasmids are shown on the next page, including important restriction sites and distances (in base pairs) between the sites