Tissue Culture a technique which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channelsTissue Culture • A technique which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium. • By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels