Question 4, continued c)Researchers are currently working on gene therapy for disease X patients. The most adenovirus is a double-stranded DNA virus that target wivip thelial cells, it can be used to promising therapy has involved incorporating the disease x gene into an adenovirus. Becau deliver the disease X gene to the lung cells of the affected individual display of MHC I molecules on the surface of cells. Why is this a desirable property of the virus used to deliver the disease x gene? ii)Using the plasmids and restriction enzymes provided, design a procedure to create a double-stranded dna to incorporate into the adenovirus particle. The final product should be linear, contain the majority of the virus genome and have the disease x gene under control of the El promoter(Pel). Nhel and Spel create the same sticky ends. All the other restriction enzymes create unique cuts P BamHI HindIll start Spel disease X cdnA BR-Ad2-7 Adenovirus pCMV-diseaseX HindII BamhI EcoRI Spring 2004 Final exam practiceSpring 2004 Final Exam Practice 7 Question 4, continued c) Researchers are currently working on gene therapy for disease X patients. The most promising therapy has involved incorporating the disease X gene into an adenovirus. Because adenovirus is a double-stranded DNA virus that targets lung epithelial cells, it can be used to deliver the disease X gene to the lung cells of the affected individual. i) The adenovirus used in these studies is able to produce gp19, a protein that inhibits the display of MHC I molecules on the surface of cells. Why is this a desirable property of the virus used to deliver the disease X gene? ii) Using the plasmids and restriction enzymes provided, design a procedure to create a, double-stranded DNA to incorporate into the adenovirus particle. The final product should be linear, contain the majority of the virus genome and have the disease X gene under control of the E1 promoter (PE1). NheI and SpeI create the same sticky ends. All the other restriction enzymes create unique cuts. pBR-Ad2-7 PE1 BamHI NheI HindIII SpeI BamHI Adenovirus genome pCMV-diseaseX EcoRI HindIII SpeI disease X cDNA start stop