DNA SU upercoilin g To package the A into the cell requires that the dna be supercoiled Proteins There are over 50 supercoiled domain domains in the Open ciro E. coli chromosome they are stabilized by association with the Supercoil structural proteins.DNA Supercoiling To package the DNA into the cell requires that the DNA be supercoiled. There are over 50 supercoiled domains in the E. coli chromosome, they are stabilized by association with the structural proteins