3. Identification and Doubling of haploid (1)Identification a) Morphology b)Physiological and biochemical characteristics c)Genetic markers d)Cytology (2) Doubling 0 Natural doubling (relatively low) b) artificial doubling50 ppm colchicine秋水仙素3. Identification and Doubling of Haploid (1) Identification a) Morphology b) Physiological and Biochemical characteristics c) Genetic markers d) Cytology (2) Doubling a) Natural doubling (relatively low) b) artificial doubling 50ppm colchicine 秋水仙素