植物表达载体的构建 RT-PCR Ligation B0w邸L BAK1 Kpx! S2 BAK1人Sacd pGbak PCAMBI NOS Term 35s poly A UBI-1 promoter LB RB BAKI Hyg GUSa BamHI Sacl Kan(R)35s prometer 35S promoter NOS Term BamHI/Sacl Ligation 图三RNAi植物表达载体TDNA区段结构图 flag Tnos hpty P35s 图二超量表达植物表达载体构建流程植物表达载体的构建