Absorbance Amide I: C=O stretching Amide il: nh bending 20001900180017001600150014001300 dary Derivative spectrum C=O---H-0 1700 1680 Wavenumber(cm)• Absorbance Amide I: C=O stretching Amide II: NH bending • Secondary Derivative spectrum --C=O---H-O 13001400150016001700180019002000 Absorbance Col 21 vs Col 22 Wavenumber (cm-1) 160016201640166016801700 d 2A/d n 2 Amide I Amide II