Carrier Hapten Antibodies to hapten Immunize rabbit Antibodies to carrier Hapten-carrier conjugate Antibodies to conjugate of hapten and carrier Injection with: Antibodies formed: Hapten(①NP) None Protein carrier (BSA) Anti-BSA Hapten-carrier Anti-DNP (major) conjugate (DNP-BSA) Anti-BSA(minor) Anti-DNP/BSA (minor)