1 Machanicalisolation 从高度液泡化的细胞, practice/this ttee fficult Cut plasmolyzed and the yield of viable tissue and protoplasts is meager. vantage, subsequent however is that the deplasmolysis deleterious effects of results in expansion the wall-degrading enzymes,plast festoon the and release of the metabolism of the protoplasts from theprotoplasts Protoplast released are cut ends of the cell eliminated1、Machanical isolation • Cut plasmolyzed tissue and subsequent deplasmolysis results in expansion and release of the protoplasts from the cut ends of the cell. • In practice this technique is difficult and the yield of viable protoplasts is meager. One advantage, however, is that the deleterious effects of the wall-degrading enzymes on the metabolism of the protoplasts are eliminated. 从高度液泡化的细胞