Proteins of DNA Replication DNA exists in the nucleus as a condensed, compact structure to prepare dna for replication a series of proteins aid in the unwinding and separation of the double-stranded DNA molecule These proteins are required because dna must be single-stranded before replication can proceed 1. DNA Helicases- These proteins bind to the double stranded DNa and stimulate the separation of the two strands 2. DNA single-stranded binding proteins- these proteins bind to the dna as a tetramer and stabilize the single-stranded structure that is generated by the action of the helicases Replication is 100 times faster when these proteins are attached to the single stranded dna 3. DNA Topoisomerase- This enzyme catalyzes the formation of negative supercoils that is thought to aid with the unwinding process In addition to these proteins, several other enzymes are involved in bacterial dNA replicationProteins of DNA Replication DNA exists in the nucleus as a condensed, compact structure. To prepare DNA for replication, a series of proteins aid in the unwinding and separation of the double-stranded DNA molecule. These proteins are required because DNA must be single-stranded before replication can proceed. 1. DNA Helicases - These proteins bind to the double stranded DNA and stimulate the separation of the two strands. 2. DNA single-stranded binding proteins - These proteins bind to the DNA as a tetramer and stabilize the single-stranded structure that is generated by the action of the helicases. Replication is 100 times faster when these proteins are attached to the single￾stranded DNA. 3. DNA Topoisomerase - This enzyme catalyzes the formation of negative supercoils that is thought to aid with the unwinding process. In addition to these proteins, several other enzymes are involved in bacterial DNA replication