10/20/2017 Serological Identification Gram-negative cell wall O-polysaccharide Aim:to determine the complete antigenic formulas for individual Salmonella isolates 3 main antigenic determinants 1.O-antigen associated with lipopolysaccharides heat stabile polysaccharide 与A ran Serological Identification 2.H-antigen associated with peritrichous flagella heat-labile protein antigens 3.Vi(virulence)-antigen associated with capsular antigen Weak or no only in serovars Readily agglutinates Typhi,Paratyphi C, with antibodies agglutination with and Dublin antibodies10/20/2017 5 Serological Identification Aim: to determine the complete antigenic formulas for individual Salmonella isolates 3 main antigenic determinants 1. O-antigen associated with lipopolysaccharides - heat stabile polysaccharide Gram-negative cell wall Readily agglutinates with antibodies Weak or no agglutination with antibodies Serological Identification 2. H-antigen associated with peritrichous flagella - heat- labile protein - may produce 1 or 2 sets of flagellar antigens 3. Vi (virulence)- antigen associated with capsular antigen - only in serovars Typhi, Paratyphi C, and Dublin