ytical biotechnology 39. Kleerekoper Q, Liu w, Choi D, Putkey JA: Identification of binding 56. Bertho G, Ghart es for bepridil and trifluoperazine on cardiac troponin C. J Biol Chem1998,27381538160. 二邮 cor tion between antibiotic 40. Otting G, wuthrich nuclear filters in two-dimensional ations. Bioorg Med Chem 1998 6209221 for studies 1990233996 entification of pr C, Legault P, Vincent SJF, Greenblatt J, Konrat R, Kay LE: eson B1995,106:195198 ex by transferred ROESY. J Magn NMR spectroscopy: application to a bacteriopha B RNA complex. J Am Chem Soc 1997. 58. Barsukov IL, Lian L-Y, Ellis J, Sze K-H. Shaw WV. Roberts GCK: The enzyme a bound to chloramphenic rase determined by transferred NOE experiments. 2. Martorell G, Gradwell M, Birdsall B, Bauer C, Frenkiel TA, J Mol Bio11996,262:543558 T, Palmer AG lll, Mc Dermott AE: Conformation dihydrofolate reductase. Biochemistry 1994, 33: 12416-12426. ar Overhauser studies, Biochemistry 1997 36:14202 ucture of a brodimoprim analog in its 60. Massefski W. Redfield us casei dihydrofolate reductase. Bio mination of multiple-step spin 34:1169011702 Johnson」 EM, Wnight JE, Pardo J, Rosowsky A, Wagner G 61. Zonai z, Juranic N, Markley JL, Macura S: Magnetization exchange NADPH ture of the antitumor compound PT523 and network editing- mathematical principles and experimental demonstration. Chem Phys 191 reductase msty199736:43994411 45. Gonnella NC, Li YC, Zhang XL, Paris CG: Bioactive conformation of "assition of spin diffusion and multidimensional NMR spectroscopy. Bioorg Med Chem 1997 46. Gradwell M, Feeney J: Validation of the use of intermolecular NOE docked structures of protein ligand at/ Acad Sci USA 1997. 9 omplexes, J Biomol NMR 1996. 7: 48-58 This paper describes the application of the QUIET-NOESY experiment to 47. Modi S, Paine ML, Sutcliffe M, Lian L-Y, Primrose wU. Wolf CR an cytochrome P450 2D6 based on homology modelling and NMR studies of substrate binding. Biochemistry 1996, 35: 4540-4550. 48. Modi S, Gilham DE, Sutcliffe M, Lian L-Y, Primrose WU. Wolf CR nyl-1, 2, 3, 6-tetrahydropyridi glycosidic bond was not, and the coexistence of two co ations about this bond in the complex is suggested 2D the presence of pound to e dis. 64. Cox Ra,ycosiasu EH: Biologically important conformations of er effect spectroscopy Ba by transferred nuclear 36:23532359 ty1997 of the paramagnetic relaxation approach in studying the specificity65 M, Paci M, Sette M, Oakley Aj, Parker MW. Lo Bello M Primrose WU, Lian L-Y, Roberts GCK o human glutathione transferase P m of cytochrome p450 BM3 involves a 6A ments with the crystal st 1996,3:41 bound substrate on reduction Nat Struct Bio/ Transferred NOE experiments lead to on of bound glutathione tion but sidechain conformations 0. Oliver CF, Modi S, Sutcliffe M, Primrose WU, Lian L-Y. Roberts GCK The origins of this difference ar A single-residue mutation of docking calculations and kinetic experiments; it is con- cluded that the NMR structure may correspond to that of a'pre-complex' 36:1567-1572 51. Pol-Scaife S, Attias R, Dansette PM, Mansuy D: The substrate mM0门hmR nergy conformation of C-lactose, a non hydrolyzable substrat nalogue. NMR and m te(e. g. the location of a putative positive charge)from the of Chem Soc1998,120:1309-1318 52. Albrand JP, Birdsall B, Feeney J, Roberts CK, Burgen ASV: The use us repla indicate that C-lactose (in which the glyco- transferred nuclear Overhauser effects in the study of th tosidase adopts a conformation which is only slightly populated (-5%)in the free ligand all molecules bound to proteins. int JBi 4 67. Montero M, Vallmitjana M, Perez-Pons JA, Querol confor cellobiose bound to a B-glucosidase from Streptomyces sp. FEBS Le1998,421:243248 982,48:402417 54. Campbell AP, Sykes BD: The two-dimensional transferred nuclear L-selectin. J Am Chem Soc 1997.119: 1727-1736 Biomol Struct 1993, 22: 99-122 55. NiF: Recent advances in transferred NOE methods. Prog NMR ed NOE experiments Spectrosc 1994 informers of the tetrasaccharide are recognised by E- and P-selectin on