procedures template dna or pre-denaturation RNA denature prlmers completel Tag enzyme, · Denaturation °10× pcr buffer, annealing g Elongation 5mmo1/L dNTPs cycles: 25-30procedures • template(DNA or RNA), • primers, • Taq enzyme, • 10×PCR buffer, • Mg++, • 5mmol/L dNTPs • pre-denaturation-- denature completely • Denaturation • annealing • Elongation • cycles:25-30