[实验步骤] 1.取材将饥饿24h的大 白鼠处死。迅速取出肝 组织浸入预冷的生理盐 水，洗去血污，滤纸吸 生理盐水 干。在培养皿中剪碎， 用少量0.25M蔗糖液洗 涤数次。 2.匀浆每组取适量剪碎 组织，放入匀浆器中， 加入3ml的0.25M蔗糖 溶液进行匀浆。 3.过滤将组织匀浆完毕 ,用滤网过滤，每组取 2ml的过滤液移入离心 管中。 This document is produced by trial version of Print2Flash.Visit www.print2flash.com for more informationThis document is produced by trial version of Print2Flash. Visit www.print2flash.com for more information